Communications in Plant Sciences, vol 9, p. 1-5, 2019 (2019001)
Comparison between extraction methods of photosynthetic pigments in Acacia mangium
Susana Cristine Siebeneichler, Jacqueline Silva Barbosa, Antônio Marcos Milhomem Cruz, Marilene Alves Dias Ramos, Hallefy Elias Fernandes and Vitor L. Nascimento*
*Correspondence to: firstname.lastname@example.org
Abstract: The aim of this work was to determine a simple and rapid protocol for the extraction of photosynthetic pigments (chlorophylls and carotenoids) from Acacia mangium (Willd.), a pioneer species with rapid growth and that has been used in commercial forests in northern Brazil. The experiment consisted of 4×5 factorial, 4 solvents (ethanol, dimethylsulfoxide (DMSO), N,N-dimethylformamide (DMF), and 80% acetone) and 5 extraction times (24, 48, 72, 96, and 120 hours). The equations used for calculations varied according to the solvent used. For a quantification of the photosynthetic pigment contents of A. mangium it is recommended to use 80% acetone with 84 hours of extraction since this solvent allows the efficient extraction of both chlorophylls and carotenoids with a single protocol.
Determination of the Acacia leaves pigments (chlorophylls and carotenoids) it is possible with a single protocol in 84 hours, using an extraction with 80% acetone.
Keywords: Acacia, Carotenoids, Chlorophylls, Extraction time, Solvents. (more…)