Communications in Plant Sciences, vol 8, p. 82-89, 2018 (2018012)
Authors: Tauane Santos Brito, Renan Pan, Elisiane Inês Dall’Oglio Chaves, and Vandeir Francisco Guimarães
Abstract: The objective of the present research was to evaluate the initial development of maize (Zea mays) inoculated with plant growth-promoting diazotrophic bacteria Azospirillum brasilense, Burkholderia ambifaria and Herbaspirillum seropedicae in vitro and in protected culture. The experiment in protected culture consisted in the comparison of four methods of nitrogen fertilizations in maize crop (absence, broadcast, placement, broadcast and placement) associated or not to inoculation with growth-promoting bacteria. Morphological parameters, foliar nitrogen content and bacterial population were evaluated at 30 and 45 days after sowing. The in vitro essay consisted in the cultivation of inoculated plants in culture medium, with and without nitrogen and then, at seven days after seeding, evaluated morphological parameters, foliar nitrogen content and bacterial population. A. brasilense was more responsive to placement fertilization, while H. seropedicae for the association between placement and broadcast fertilization, or else the absence of fertilization. Yet, B. ambifaria was responsible to the absence of fertilization. Bacterial populations in protected culture varied according to the fertilization used and the period of collection. In vitro, the bacterial population was higher epiphytically.
1) A. brasilense is more responsive to placement fertilization, and B. ambifaria to the absence of fertilization. H. seropedicae is more responsive the association between placement and broadcast fertilization, or else the absence of fertilization.
2) Bacterial populations vary according to the fertilization and the period of collection in protected culture, and they are higher epiphytically in vitro.
Keywords: Zea mays, Azospirillum brasilense, Burkholderia ambifaria, Herbaspirillum seropedicae, Diazotrophic bacteria. (more…)